Review



the promoterless luciferase reporter vector pgl2 basic dna  (Promega)

 
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    Structured Review

    Promega the promoterless luciferase reporter vector pgl2 basic dna
    The Promoterless Luciferase Reporter Vector Pgl2 Basic Dna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl2+basic+vector+dna/pgl3+basic/pm09162073-24-11-35
    Average 90 stars, based on 1 article reviews
    the promoterless luciferase reporter vector pgl2 basic dna - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Hyperpolarization-activated cyclic nucleotide-gated cation channel 3 promotes HCC development in a female-biased manner
    Article Snippet: The fraction was cloned into the pGL2-Basic vector (Promega) to get the pGL2-promoHCN3.

    Article Title: A Novel Role for GADD45 β as a Mediator of MMP-13 Gene Expression during Chondrocyte Terminal Differentiation
    Article Snippet: The PCRs were carried out using the Pfu Turbo DNA polymerase (Stratagene), and the products were subcloned in the pGL2-basic vector (Promega, Madison WI) and verified by DNA sequencing.

    Article Title: Smad1 and its target gene Wif1 coordinate BMP and Wnt signaling activities to regulate fetal lung development
    Article Snippet: A region of the Wif1 gene (−940 to +20) was amplified from mouse genomic DNA and subcloned into the pGL2-Basic vector (Promega) (Wif1-luc) and verified by DNA sequencing.

    Article Title: A Conserved E2F6-Binding Element in Murine Meiosis-Specific Gene Promoters
    Article Snippet: The Takara DNA ligation kit was then used to ligate the fragment of the Slc25a31 promoter into a PGL2-basic vector (Promega) containing a luciferase reporter gene.

    Article Title: A Novel Role for GADD45β as a Mediator of MMP-13 Gene Expression during Chondrocyte Terminal Differentiation
    Article Snippet: The PCRs were carried out using the Pfu Turbo DNA polymerase (Stratagene), and the products were subcloned in the pGL2-basic vector (Promega, Madison WI) and verified by DNA sequencing.

    Article Title: Signal Transduction Pathways Mediating Neurotensin-stimulated Interleukin-8 Expression in Human Colonocytes
    Article Snippet: The sequences were confirmed by DNA sequencing using primers specific for the pGL2-basic luciferase expression vector (GL primers 1 and 2; Promega Corp., Madison, WI).

    Article Title: Random, Asynchronous, and Asymmetric Transcriptional Activity of Enhancer-Flanking Major Immediate-Early Genes ie1/3 and ie2 during Murine Cytomegalovirus Latency in the Lungs
    Article Snippet: The resulting 1.328-kbp fragment containing mCMV sequences from map positions 184,272 to 182,944 was cloned into the Sma I-digested pGL2-Basic vector (Promega; GenBank accession no. 47295) that contains the Firefly luciferase gene.

    Sequencing:

    Article Title: A functional variant of the adipocyte glycerol channel aquaporin 7 gene is associated with obesity and related metabolic abnormalities.
    Article Snippet: .. A 1,720-bp fragment of human AQP7 promoter ( 11/ 1,731, according to the previously reported sequence numbering [18]) containing the 953A variant, in haplotypic combination with the major allele of the SNP rs3758269 (i.e., 1123C), was amplified from genomic DNA and inserted in the pGL2 basic luciferase expression vector (Promega) (Fig. 2, left panel). .. The 953G mutant promoter vector was obtained using the QuickChange Site-Directed Mutagenesis kit (Stratagene).

    Variant Assay:

    Article Title: A functional variant of the adipocyte glycerol channel aquaporin 7 gene is associated with obesity and related metabolic abnormalities.
    Article Snippet: .. A 1,720-bp fragment of human AQP7 promoter ( 11/ 1,731, according to the previously reported sequence numbering [18]) containing the 953A variant, in haplotypic combination with the major allele of the SNP rs3758269 (i.e., 1123C), was amplified from genomic DNA and inserted in the pGL2 basic luciferase expression vector (Promega) (Fig. 2, left panel). .. The 953G mutant promoter vector was obtained using the QuickChange Site-Directed Mutagenesis kit (Stratagene).

    Amplification:

    Article Title: A functional variant of the adipocyte glycerol channel aquaporin 7 gene is associated with obesity and related metabolic abnormalities.
    Article Snippet: .. A 1,720-bp fragment of human AQP7 promoter ( 11/ 1,731, according to the previously reported sequence numbering [18]) containing the 953A variant, in haplotypic combination with the major allele of the SNP rs3758269 (i.e., 1123C), was amplified from genomic DNA and inserted in the pGL2 basic luciferase expression vector (Promega) (Fig. 2, left panel). .. The 953G mutant promoter vector was obtained using the QuickChange Site-Directed Mutagenesis kit (Stratagene).

    Luciferase:

    Article Title: A functional variant of the adipocyte glycerol channel aquaporin 7 gene is associated with obesity and related metabolic abnormalities.
    Article Snippet: .. A 1,720-bp fragment of human AQP7 promoter ( 11/ 1,731, according to the previously reported sequence numbering [18]) containing the 953A variant, in haplotypic combination with the major allele of the SNP rs3758269 (i.e., 1123C), was amplified from genomic DNA and inserted in the pGL2 basic luciferase expression vector (Promega) (Fig. 2, left panel). .. The 953G mutant promoter vector was obtained using the QuickChange Site-Directed Mutagenesis kit (Stratagene).

    Expressing:

    Article Title: A functional variant of the adipocyte glycerol channel aquaporin 7 gene is associated with obesity and related metabolic abnormalities.
    Article Snippet: .. A 1,720-bp fragment of human AQP7 promoter ( 11/ 1,731, according to the previously reported sequence numbering [18]) containing the 953A variant, in haplotypic combination with the major allele of the SNP rs3758269 (i.e., 1123C), was amplified from genomic DNA and inserted in the pGL2 basic luciferase expression vector (Promega) (Fig. 2, left panel). .. The 953G mutant promoter vector was obtained using the QuickChange Site-Directed Mutagenesis kit (Stratagene).

    Plasmid Preparation:

    Article Title: A functional variant of the adipocyte glycerol channel aquaporin 7 gene is associated with obesity and related metabolic abnormalities.
    Article Snippet: .. A 1,720-bp fragment of human AQP7 promoter ( 11/ 1,731, according to the previously reported sequence numbering [18]) containing the 953A variant, in haplotypic combination with the major allele of the SNP rs3758269 (i.e., 1123C), was amplified from genomic DNA and inserted in the pGL2 basic luciferase expression vector (Promega) (Fig. 2, left panel). .. The 953G mutant promoter vector was obtained using the QuickChange Site-Directed Mutagenesis kit (Stratagene).



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